This instrument uses MAPSS technology to perform cell by cell analysis from a single dilution to enumerate and differentiate White Blood Cells and provide the performance needed for patient samples. Additionally, the instrument uses optical laser light scatter analysis, without the need to reflex to another test mode, to provide both the Red Blood Cell and Platelet counts. Fluorescent NRBC and optical Platelet counts are provided first pass with no need to repeat the sample in a different test selection. Simple, automated, single mode calibration makes calibrating the analyzer a quick and easy operation.
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Search Menu Abstract The enumeration and identification of blood cells in body fluids offers important information for the diagnosis and treatment of various medical conditions. We evaluated the analytic and clinical performance of the Cell-Dyn Sapphire hematology analyzer Abbott Diagnostics Division, Santa Clara, CA for automated blood cell counting and leukocyte differential counting in cerebrospinal fluid, serous fluid peritoneal and pleural fluid , and continuous ambulatory peritoneal dialysis fluid, and we compared the performance with the respective manual methods.
In the present article, we describe its applicability for the distinct body fluids, and we highlight limitations and caveats. The enumeration and identification of blood cells in body fluids offers important information for the diagnosis and treatment of various medical conditions.
Elevated WBC counts in cerebrospinal fluid CSF are seen in meningitis, encephalitis, and other neurologic or neoplastic disorders. RBC counts are important for the diagnosis of intracerebral hemorrhage and for the exclusion of a traumatic tap as the cause of an elevated WBC count.
Apart from the organizational and inherent economic disadvantages, the manual method also has poor precision and large interobserver variability. Yet, several attempts to use automated hematology analyzers for this purpose have been described over the years.
However, manual hemacytometer counts could even be more imprecise at low cell concentrations. RBC and WBC counts were compared with the conventional manual hemacytometer counts, whereas leukocyte differential counts were compared with microscopic counts of cytocentrifuged preparations. Materials and Methods Patient Samples For method comparison, we used CSF, serous fluid 61 pleural and 40 peritoneal , and CAPD fluid samples that were collected for routine diagnostic purposes.
All samples were collected into sterile tubes without anticoagulants. First, all requested tests were performed. When the results were completely determined following standard operating procedures, the RBC count, WBC count, and WBC differential count were performed on the leftover samples, provided there was a sufficient amount of residual sample.
The analyses were performed as soon as possible and for CSF, always within 1 hour from the time of sample collection. Experienced observers examined at least nucleated cells. Cell-Dyn Sapphire Hematology Analyzer The Cell-Dyn Sapphire is an automated hematology analyzer designed for counting peripheral blood cells in whole blood samples.
The counting and differentiation principles are based on an optical and an impedance channel. For assessing the differential count, the percentages of lymphocytes and monocytes determined by the Cell-Dyn Sapphire were added, and this sum was compared with the mononuclear cell MNC count found on the cytocentrifuged preparations, which included lymphocytes, monocytes, and, in some samples, epithelial cells and macrophages.
We investigated whether these limits can be modified to make them applicable for body fluids with much lower cell concentrations compared with blood. Carryover Because the Cell-Dyn Sapphire is normally running blood samples with higher cell concentrations than in body fluids, carryover is an important issue. After a normal blood sample was analyzed, we determined the RBC and WBC count in a series of 6 tubes containing sodium chloride buffer only. Linearity Linearity was determined on serial sample dilutions prepared at relevant linearity ranges.
All samples were measured twice, and the means were compared with the expected cell counts. Different cell concentrations were prepared, and 10 aliquots of each cell concentration were counted. Within-run imprecision of the microscopic NC count was also investigated at low cell counts. It was determined from the precision study, which generated a mean and an SD for each cell concentration. Each dilution was examined 10 times. Statistical Analysis Statistical analyses were performed with Analyse-it software, version 1.
Linearity of the automated method was assessed by using linear regression analysis. Agreement between the automated cell counts and the microscopic data was examined by Passing-Bablok regression analysis.
Wilcoxon tests for paired samples were used because normal distribution could not be demonstrated. A P value of less than. Clinical performance studies were performed using Bayesian statistics.
Carryover Evaluation of carryover from normal high cell count blood samples to low cell count body fluid samples indicated that after analysis of a blood sample, at least 1 blank sample should be run before the WBC count and the RIC and ROC drop to the background level data not shown. Linearity Linearity data are shown in Table 1. Table 1.
Cell Dyn Sapphire
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