Professor Freifelder has also been a visiting scholar at the Weizman Institute and Stanford University. Freifelder was Professor of Biochemistry at Brandeis Unversity. Arvind marked biocuemistry as to-read Aug 05, Want to Read saving…. Protein adsorption Joseph D. Subhobroto Mukherjee marked it as to-read Dec 26, Nalini added it Aug 29, Just a moment while we dsvid you in to your Goodreads account. Arpita Bose marked it physiczl to-read Aug 27, Prajakta marked it as to-read Aug 28, Tuhin rated it really liked it Jul 22, Jez added it Feb 13, Vinuthatalwar added it Sep 15, Sam Dar rated it it was amazing Feb 17, Prem rated it it was amazing Mar 08, Tanya rated it really liked it Jan 11, Applications to Biochemistry and Freidelder Biology David Freifelder MacmillanAug 15, — Medical — pages 1 Review Suitable for advanced undergraduate and graduate students in biochemistry, this book provides clear, concise, well-exampled descriptions of the physical methods that biochemists and molecular biologists use.

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Physical biochemistry. Applications to biochemistry and molecular biology

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As Dr Freifelder points out, there is a gap between the theoretical analyses of such techniques in textbooks of physical biochemistry, and the practical details in manuals usually relating to particular instruments. In this attempt to fill this gap, he avoids the mathematical derivations of formulae, concentrates on specifying the essential principles of each technique, indicates the sort of apparatus used, and gives examples of its application. The emphasis is on characterization of macromolecules, and the examples are taken predominantly from studies on nucleic acids, rather than the more usual applications to proteins. The book gives a comprehensive coverage of techniques including: direct observation by light and electron microscopy; general laboratory methods, with a good outline of radioactive counting; separation methods, particularly chromatography; hydrodynamic methods, emphasizing sedimentation techniques; and spectroscopic methods, which include absorbance, fluorescence, optical rotation and nuclear magnetic resonance. A discussion of X-ray crystallography is omitted on the reasonable grounds that, although it has made major contributions to the subject, it is too specialized for inclusion in the techniques selected. Within its terms of reference, this is an admirable book that should meet its specified aim of giving students an understanding of technical applications, and make the research literature somewhat more intelligible to the uninitiated.


Physical biochemistry


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