ASTM E2149 - 10 PDF

Significance and Use 4. This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period. The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. One-hour contact time in a buffer solution allows for metabolic stasis in the population. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.

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Microchem Laboratory finds the method to be far fetched as a model system, but we conduct it regularly anyway. The concentration of the test microorganism is standardized. The microbial culture is diluted in a sterile buffer solution.

For each product to be tested, 50 mL of the standardized microbial culture is placed into 3 containers sterile glass jars work well. One jar receives only bacterial suspension, another receives the antimicrobial test substance 1g or 4in2 , and the last receives a control object an untreated object substantially similar to the test object, without the active ingredient. Microbial concentration from the bacterial suspension only jars are enumerated at "time zero.

Concentration of microorganisms in the jar that contained the antimicrobial product are compared to either the jar that contained only microbial suspension or the jar that contained the untreated control, depending on certain circumstances specified by the method. A Solution Test is conducted per the method to determine leaching of the antimicrobial agent.

The ASTM E method is written to allow experimental flexibility, which allows researchers to adapt the method to test antimicrobial products of various shapes and sizes. Weaknesses of the ASTM E Test Method Although this method is routinely used by manufacturers of antimicrobial surfaces, it is removed from actual usage of most antimicrobial products.

The types of products commonly tested by this method include antimicrobial countertops, antimicrobial toilet seats, antimicrobial pens, or antimicrobial films. An antimicrobial surface that "passes" at one hour of contact is almost certainly more effective than a surface that "passes" at 24 hours contact.

There is no clear criteria set by the method with respect to what constitutes "antimicrobial activity. One aspect of testing " treated articles " which are normally exempt from EPA data review is that there is ample opportunity to develop scientifically defensible and realistic test models to verify antimicrobial efficacy. Microchem can help speed your project to completion. Quick Contact.

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ASTM E2149-13a

Kigalkis Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. Please sign sstm an account with a subscription to this content, or email for help at support madcad. This test method is 2e intended to determine efficacy as described in 4. To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required. If an antimicrobial agent that is shown to be removed from the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration.

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ASTM E2149

Digor This test method is only intended to determine efficacy as described in 4. The electronic file may not be reproduced e any way. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism. Active view current version of standard. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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ASTM E2149:10

This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period. The susceptibility of the species to particular biocides could be altered depending on its life stage cycle. One-hour contact time in a buffer solution allows for metabolic stasis in the population. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism. For example, water soluble antimicrobials will be prone to removal from the test surface using the method described in Section 4. This test method is only intended to determine efficacy as described in 4.

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